MSC-derived exosomes protect auditory hair cells from neomycin-induced damage via autophagy regulation

Background Sensorineural hearing loss (SNHL) poses a major threat to both physical and mental health; however, there is still a lack of effective drugs to treat the disease. Recently, novel biological therapies, such as mesenchymal stem cells (MSCs) and their products, namely, exosomes, are showing promising therapeutic potential due to their low immunogenicity, few ethical concerns, and easy accessibility. Nevertheless, the precise mechanisms underlying the therapeutic effects of MSC-derived exosomes remain unclear. Results Exosomes derived from MSCs reduced hearing and hair cell loss caused by neomycin-induced damage in models in vivo and in vitro. In addition, MSC-derived exosomes modulated autophagy in hair cells to exert a protective effect. Mechanistically, exogenously administered exosomes were internalized by hair cells and subsequently upregulated endocytic gene expression and endosome formation, ultimately leading to autophagy activation. This increased autophagic activity promoted cell survival, decreased the mitochondrial oxidative stress level and the apoptosis rate in hair cells, and ameliorated neomycin-induced ototoxicity. Conclusions In summary, our findings reveal the otoprotective capacity of exogenous exosome-mediated autophagy activation in hair cells in an endocytosis-dependent manner, suggesting possibilities for deafness treatment.

Effects of high-intensity interval training on the anaerobic capacity of wrestlers

ABSTRACT Introduction Since the Wingate anaerobic experiment was proposed in the 1970s, it has come to be used as an important detection method to evaluate athletes' high-power sport capacity, the effect of training, and the training method. Therefore, it is often used to measure and evaluate the anaerobic work capacity of the human body. Objective Analyze the effects of high-intensity interval training on the anaerobic capacity of male wrestlers. Methods Professional wrestlers from a sports college were selected, as well as 30 college students majoring in physical education at a sports college, to compare the anaerobic power test. Results When evaluated by the Wingate anaerobic experiment, the value level of anaerobic power of training effects is best reflected in the first 10 s of the wrestling anaerobic experiment. The 30 s Wingate mainly reflects the effect of wrestling training on human anaerobic power, but the evaluation of anaerobic capacity from this period was not evidenced. Conclusion The Wingate anaerobic experiment is an important method for evaluating the level of wrestling training and can be used as a basis for evaluating the effect of training and the level of wrestling. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução Desde que o experimento anaeróbico Wingate foi proposto nos anos 70, ele passou a ser utilizado como um importante método de detecção para avaliar a capacidade esportiva de alta potência dos atletas, o efeito do treinamento e o método de treinamento. Com isso, ele é frequentemente usado para medir e avaliar a capacidade de trabalho anaeróbico do corpo humano. Objetivo Analisar os efeitos do treinamento intervalado de alta intensidade sobre a capacidade anaeróbica dos lutadores livres masculinos. Métodos Foram selecionados lutadores profissionais de uma faculdade de esportes, além de 30 estudantes universitários graduados em educação física em uma faculdade de esportes para comparar o teste de potência anaeróbica. Resultados Quando avaliado pelo experimento anaeróbico Wingate, o nível de valor da potência anaeróbica dos efeitos do treinamento é melhor refletido nos primeiros 10 segundos de luta livre do experimento anaeróbico. O Wingate de 30 segundos reflete principalmente o efeito do treinamento de luta livre na potência anaeróbica humana, mas a avaliação da capacidade anaeróbica desde período não foi evidenciada. Conclusão O experimento anaeróbico Wingate é um método importante para avaliar o nível de treinamento da luta livre, podendo ser utilizado como base para avaliar o efeito do treinamento e o nível da luta livre. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción Desde que se propuso el experimento anaeróbico de Wingate en los años 70, comenzó a utilizarse como un importante método de detección para evaluar la capacidad deportiva de alta potencia de los atletas, el efecto del entrenamiento y el método de entrenamiento. Por ello, se utiliza con frecuencia para medir y evaluar la capacidad de trabajo anaeróbico del cuerpo humano. Objetivo Analizar los efectos del entrenamiento interválico de alta intensidad sobre la capacidad anaeróbica de los luchadores masculinos. Métodos Se seleccionaron luchadores profesionales de una escuela de deportes, además de 30 estudiantes universitarios graduados en educación física en una escuela de deportes para comparar la prueba de potencia anaeróbica. Resultados Cuando se evalúa mediante el experimento anaeróbico de Wingate, el nivel de valor de la potencia anaeróbica de los efectos del entrenamiento se refleja mejor en los primeros 10 segundos del experimento anaeróbico de lucha. El Wingate de 30 segundos refleja principalmente el efecto del entrenamiento de lucha en la potencia anaeróbica humana, pero no se evidenció la evaluación de la capacidad anaeróbica a partir de este periodo. Conclusión El experimento anaeróbico de Wingate es un método importante para evaluar el nivel de entrenamiento de lucha, y puede utilizarse como base para evaluar el efecto del entrenamiento y el nivel de lucha. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Consequences of lower limb strength training on jump performance in athletes of martial arts / Consequências do treinamento de força nos membros inferiores sobre a capacidade de salto em atletas de artes marciais / Consecuencias del entrenamiento de fuerza de los miembros inferiores en la capacidad de salto de atletas de artes marciales

ABSTRACT Introduction: Chinese martial art has a wide and deep history, and its routine passes are a particular artistic presentation. In exhibitions, choreography with jumping is highly appreciated. Empirically, it is believed that additional strengthening of the lower limbs may promote better results in the performances, but there are no reports in the scientific literature about such a statement. Objective: Verify the consequences of lower limb strength training on jumping ability in martial arts athletes. Methods: 30 first and second tier athletes from the professional women's team of the Wushu Sports Management Center and Sports Bureau were selected as volunteers for the experimental research. The experiment lasted for three months. The characteristics of the development and change of strength of each muscle group in the knee joint of the athletes' lower limb during the experimental training were detailed, and the commonly used means of lower limb strength training were found. Results: In strength training, the best means found were skipping steps and rapidly changing direction. The correlation coefficient between jumping and strength was 0.75. Conclusion: After the experiment, the maximum torque and the average power of the lower limb knee muscle groups of the three experimental groups increased in different ranges, which shows that the strength quality of the knee joint of the athletes increased during the suggested training. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução: A arte marcial chinesa tem uma ampla e profunda história, e seus passes de rotina são uma apresentação artística em particular. Nas exibições, a coreografia com saltos é bastante apreciada. Empiricamente, acredita-se que o fortalecimento adicional de membros inferiores possa promover melhores resultados nas apresentações, porém não há relatos na literatura científica sobre tal afirmação. Objetivo: Verificar as consequências do treinamento de força nos membros inferiores sobre a capacidade de salto em atletas de artes marciais. Métodos: 30 atletas de primeira e segunda linha da equipe profissional feminina do Wushu Sports Management Center e do Sports Bureau foram selecionadas como voluntárias para a pesquisa experimental. O experimento teve duração de três meses. Foram detalhadas as características do desenvolvimento e alteração da força de cada grupo muscular na articulação do joelho do membro inferior das atletas durante o treino experimental, e foram encontrados os meios comumente utilizados de treino de força dos membros inferiores. Resultados: No treino de força, os melhores meios encontrados foram saltar passos e alterar rapidamente de direção. O coeficiente de correlação entre salto e força foi de 0,75. Conclusão: Após o experimento, o torque máximo e a potência média dos grupos musculares do joelho dos membros inferiores dos três grupos experimentais aumentaram em diferentes faixas, o que mostra que a qualidade de força da articulação do joelho das atletas aumentou durante o treino sugerido. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción: El arte marcial chino tiene una amplia y profunda historia, y sus pases de rutina son una presentación artística particular. En las exhibiciones, la coreografía con saltos es muy apreciada. Empíricamente, se cree que el fortalecimiento adicional de los miembros inferiores puede promover mejores resultados en las actuaciones, pero no hay informes en la literatura científica sobre tal afirmación. Objetivo: Verificar las consecuencias del entrenamiento de la fuerza de los miembros inferiores sobre la capacidad de salto en atletas de artes marciales. Métodos: 30 atletas de primera y segunda línea del equipo profesional femenino del Centro de Gestión Deportiva de Wushu y de la Oficina de Deportes fueron seleccionados como voluntarios para la investigación experimental. El experimento duró tres meses. Se detallaron las características del desarrollo y el cambio de la fuerza de cada grupo muscular en la articulación de la rodilla de la extremidad inferior de los atletas durante el entrenamiento experimental, y se encontraron los medios utilizados habitualmente para el entrenamiento de la fuerza de la extremidad inferior. Resultados: En el entrenamiento de fuerza, los mejores medios encontrados fueron saltar pasos y cambiar rápidamente de dirección. El coeficiente de correlación entre los saltos y la fuerza fue de 0,75. Conclusión: Tras el experimento, el par máximo y la potencia media de los grupos musculares de la rodilla de los miembros inferiores de los tres grupos experimentales aumentaron en diferentes rangos, lo que demuestra que la calidad de la fuerza de la articulación de la rodilla de los atletas aumentó durante el entrenamiento propuesto. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Prevalence of subhealth status and its effects on mental health and smartphone addiction: a cross-sectional study among Chinese medical students

SUMMARY OBJECTIVE: This study aimed to investigate the suboptimal health status or subhealth status and their relationship with mental health and smartphone addiction among Chinese medical students. METHODS: A cross-sectional survey was conducted at Wannan Medical College of China in Wuhu. RESULTS: A total of 2,741 students were surveyed in October 2020. Of 2,741 Chinese medical students who completed the survey, 904 (33%) participants reported to have had subhealth status. Anxiety status (p<0.001), depression status (p<0.001), and smartphone addiction status (p<0.001) have strong association with subhealth status. CONCLUSION: This survey shows that the detection rate of subhealth status in Chinese medical students was 33%. Anxiety, depression, and smartphone addiction students had a higher detection rate of subhealth status. The anxiety, depression, and smartphone addiction of Chinese medical students are associated with subhealth status.

Effects of medications on incidence and risk of knee and hip joint replacement in patients with osteoarthritis: a systematic review and meta-analysis

Abstract Background: This systematic review and meta-analysis aimed to investigate the incidence and risk of knee and hip replacement in patients with osteoarthritis (OA) treated with different medications. Methods: OVID MEDLINE, OVID EMBASE, Cochrane and Web of Science electronic databases were searched from inception to May 4th, 2022. Clinical trials, including randomized controlled trials, cohort studies and case-control studies, were selected. The meta-analysis effect size was estimated using either incidence with 95% confidence intervals (CIs) or odds ratio (OR)/relative risk (RR) with 95% CIs. The risk of bias and heterogeneity among studies were assessed and analyzed. Results: Forty studies were included, involving 6,041,254 participants. The incidence of joint replacement in patients with OA varied according to the study design and treatments. The incidence of knee arthroplasty varied from 0 to 70.88%, while the incidence of hip arthroplasty varied from 11.71 to 96.43%. Compared to non-users, bisphosphonate users had a reduced risk of knee replacement (RR = 0.71, 95% CI: 0.66-0.77; adjusted hazard ratio [aHR] = 0.76, 95% CI: 0.70-0.83). Compared to intra-articular corticosteroid users, hyaluronic acid (HA) users had a higher risk of knee arthroplasty (RR = 1.76, 95% CI: 1.38-2.25). No publication bias was observed. Conclusion: Bisphosphonate treatment is associated with a reduced risk of knee replacement. More studies are needed to validate our results due to the limited number of eligible studies and high heterogeneity among studies.

Dexmedetomidine pretreatment alleviates cerebral ischemia/reperfusion injury by inhibiting neuroinflammation through the JAK2/STAT3 pathway

Dexmedetomidine (DEX) is known to provide neuroprotection against cerebral ischemia and reperfusion injury (CIRI), but the exact mechanisms remain unclear. This study was conducted to investigate whether DEX pretreatment conferred neuroprotection against CIRI by inhibiting neuroinflammation through the JAK2/STAT3 signaling pathway. Middle cerebral artery occlusion (MCAO) was performed to establish a cerebral ischemia/reperfusion (I/R) model. Specific-pathogen-free male Sprague-Dawley rats were randomly divided into Sham, I/R, DEX, DEX+IL-6, and AG490 (a selective inhibitor of JAK2) groups. The Longa score, TTC staining, and HE staining were used to evaluate brain damage. ELISA was used to exam levels of TNF-α. Western blotting was used to assess the levels of JAK2, phosphorylated-JAK2 (p-JAK2), STAT3, and phosphorylated-STAT3 (p-STAT3). Our results suggested that both pretreatment with DEX and AG490 decreased the Longa score and cerebral infarct areas following cerebral I/R. After treatment with IL-6, the effects of DEX on abrogating these pathological changes were reduced. HE staining revealed that I/R-induced neuronal pathological changes were attenuated by DEX application, consistent with the AG490 group. However, these effects of DEX were abolished by IL-6. Furthermore, TNF-α levels were significantly increased in the I/R group, accompanied by an increase in the levels of the p-JAK2 and p-STAT3. DEX and AG490 pretreatment down-regulated the expressions of TNF-α, p-JAK2, and p-STAT3. In contrast, the down-regulation of TNF-α, p-JAK2, and p-STAT3 induced by DEX was reversed by IL-6. Collectively, our results indicated that DEX pretreatment conferred neuroprotection against CIRI by inhibiting neuroinflammation via negatively regulating the JAK2/STAT3 signaling pathway.

Application of machine learning in the prediction of college students suicidal ideation / 中国学校卫生

Objective@#To explore the predictive effect of machine learning algorithms on college students suicidal ideation and to analyze the associated factors of college students suicidal ideation.@*Methods@#The mental health data of 21 224 undergraduates was selected from a university in 2021. The independent variables were 37 demographic and internal and external mental health factors. The dependent variable was whether college students had suicidal ideation. Support vector machine, random forest and LightGBM algorithm were used to establish prediction models. The model was used in test set to so as to evaluate the model s prediction effect by using detection rate, F1 score and accuracy rate. Based on the superior model, the highrisk factors of suicidal ideation in college students were analyzed.@*Results@#The detection rates of support vector machine, random forest, and LightGBM models were 61.0% ,64.0%, 69.0%; F1 scores were 0.63, 0.63, 0.64, and accuracy rates were 73.0%, 73.0%, 72.0%, respectively. Based on the superior LightGBM model, risk factors of suicidal ideation in college students included, depression, grade, gender, despair, place of origin, sense of meaning, attitude toward suicide, dependence, family economic situation, hallucinatory delusion symptoms, anxiety, internet addiction, and interpersonal distress.@*Conclusion@#The LightGBM model has a better prediction effect than the support vector machine and random forest models.

Application of machine learning in the prediction of college students suicidal ideation / 中国学校卫生

Objective@#To explore the predictive effect of machine learning algorithms on college students suicidal ideation and to analyze the associated factors of college students suicidal ideation.@*Methods@#The mental health data of 21 224 undergraduates was selected from a university in 2021. The independent variables were 37 demographic and internal and external mental health factors. The dependent variable was whether college students had suicidal ideation. Support vector machine, random forest and LightGBM algorithm were used to establish prediction models. The model was used in test set to so as to evaluate the model s prediction effect by using detection rate, F1 score and accuracy rate. Based on the superior model, the highrisk factors of suicidal ideation in college students were analyzed.@*Results@#The detection rates of support vector machine, random forest, and LightGBM models were 61.0% ,64.0%, 69.0%; F1 scores were 0.63, 0.63, 0.64, and accuracy rates were 73.0%, 73.0%, 72.0%, respectively. Based on the superior LightGBM model, risk factors of suicidal ideation in college students included, depression, grade, gender, despair, place of origin, sense of meaning, attitude toward suicide, dependence, family economic situation, hallucinatory delusion symptoms, anxiety, internet addiction, and interpersonal distress.@*Conclusion@#The LightGBM model has a better prediction effect than the support vector machine and random forest models.

Effect of empowerment educational program on AIDS prevention and treatment in one university / 中国学校卫生

Objective@#To apply and evaluate the effect of empowerment educational program on AIDS prevention and treatment among freshmen in one university.@*Methods@#The method of two stage stratified sampling was used to select the experimental and control group. The traditional health education was implemented among the control group, and the empowerment education was implemented for the experimental group. The effect of the two groups was compared before and after intervention.@*Results@#For experimental group, the awareness rate of AIDS(65.02%) ( χ 2=61.02， P <0.01) and the overall score of attitude and behavior(16.71± 2.53 )( t =-2.66， P <0.05) were significantly improved after intervention(82.96%，18.58±1.95). For the control group, there was significant difference in awareness rate of AIDS after intervention(67.70% vs 96.02%， χ 2=18.64, P <0.05), while there was no statistical difference in overall score of attitude and behavior after intervention(16.52±1.50 vs 17.16±1.57， t =-1.51, P =0.14). There was no significant difference in awareness rate between the two groups before intervention ( χ 2=0.36, P =0.55), but there was a statistical difference after intervention ( χ 2=20.42, P <0.01). There was statistical difference in attitude and behavior scores between the two groups after intervention ( P <0.05).@*Conclusion@#Empowerment educational program can improve the awareness rate of AIDS among college students, help to establish an objective attitude towards AIDS and infected patients, and to reduce high risk sexual behavior, also it is more effective compared to traditional education method.

COVID-19 vaccine hesitancy among Chinese residents under the free vaccination policy

SUMMARY OBJECTIVE: This study aimed to assess the attitudes of Chinese residents toward COVID-19 vaccines and explore the potential drivers for Chinese residents' vaccine hesitancy. METHODS: A cross-sectional survey was conducted from February 16 to March 16, 2021, by administering an online questionnaire to the Chinese residents. RESULTS: Of 5240 residents who completed the survey, 464 (8.9%) participants reported to have had one shot, and 348 (6.6%) reported to have had 2 shots. At the time the questionnaire was administered, 2298 (43.9%) participants reported they wanted to get vaccinated, while 2255 (43.0%) declared that they still did not know, and 687 (13.1%) respondents declared vaccine refusal. Overall, 2255 (43%) participants were categorized as vaccine hesitancy. Female participants (p=0.000), <20 years old (p=0.000), have low risk of COVID-19 (p=0.000) infection and strong associations of vaccine hesitancy. eHealth literacy was a protective factor. CONCLUSIONS: The results of this study show high rates of vaccine hesitancy in China. This could pose a serious threat to the preventive measures that aimed at controlling COVID-19 spread in the country. The government and different media platforms should encourage the dissemination of correct information about vaccines, the communities and medical staff to improve residents' knowledge about vaccines, and strive to improve residents' electronic health literacy.

Suboptimal health status of nurses in Wuhan, China during the COVID-19 outbreak

SUMMARY OBJECTIVE: This study analyzes the suboptimal health status (SHS) and influencing factors of nurses in Wuhan Hospital, China during the coronavirus disease 2019 (COVID-19) outbreak. METHODS: This study was conducted through an online survey, from March 1-7, 2020, in Wuhan, China. The data collection tools, such as Suboptimal Health Status Questionnaires, Generalized Anxiety Disorder, and Chinese version of the Perceived Stress Scale, were used. RESULTS: The average value of suboptimal health status was 28.44 (standard deviation=15.15). The overall prevalence of SHS was 35.1%. Suboptimal health status of the nurses was significantly different based on their gender, age, whether they directly care for COVID-19 patients, anxiety level, and stress perception expect education. Multivariate analysis found that average sleep times per day, female, age, directly participate in the rescue of COVID-19, self-infection, and anxiety were the influencing factors of suboptimal health status. CONCLUSIONS: First-line nurses have poor suboptimal health status in Wuhan.

lncRNA LINC01410 regulates the proliferation, apoptosis and temozolomide sensitivity of glioma A172 cells via miR-205-5p / 中国肿瘤生物治疗杂志

@#[摘 要] 目的：探讨长链非编码RNA（lncRNA）LINC01410对胶质瘤A172细胞增殖、凋亡和替莫唑胺（temozolomide, TMZ）敏感性的影响及其机制。方法: 用qPCR法检测胶质瘤细胞系H4、SHG-44、A172和正常星形胶质细胞HA1800中LINC01410表达水平。将LINC01410 shRNA、shRNA control和miR-205-5p 抑制剂（inhibitor）、inhibitor control转染至A172细胞，MTT法、流式细胞术分别检测400 μmol/L TMZ处理后，转染细胞的增殖活性和凋亡水平，WB法检测细胞中Bax、Bcl-2、cyclin D1、p27的表达。在线生物信息学软件LncBase分析LINC01410的靶基因，双荧光素酶报告基因实验验证LINC01410与miR-205-5p的靶向关系。结果: LINC01410在3种胶质瘤细胞中的表达水平均显著高于正常星形胶质细胞HA1800（均P<0.01），以在A172细胞中的表达水平最高（P<0.01）。转染LINC01410 shRNA和TMZ处理后，A172细胞的增殖能力下降、G1期细胞比例和凋亡率均升高（均P<0.01），细胞中Bax、p27表达水平升高而Bcl-2、cyclin D1表达水平下降（均P<0.01）。双荧光素酶报告基因实验证实LINC01410靶向结合miR-205-5p，下调LINC01410促进miR-205-5p表达。转染miR-205-5p抑制剂可逆转下调LINC01410和TMZ处理对A172细胞增殖、周期和凋亡的影响。结论: 下调lncRNA LINC01410可抑制胶质瘤A172细胞增殖、阻滞细胞周期、诱导细胞凋亡且提高对TMZ敏感性，其发生机制似与LINC01410对miR-205-5p的靶向作用有关。

Public practice，attitude and knowledge of coronavirus disease / 热带医学杂志

@#Objective To analyze the status of knowledge，attitude and practice of the coronavirus disease（COVID ⁃19）among the adults of 18⁃59 years old in China，and to provide scientific basis for corresponding health education strategies. Methods In the rapid development phrase of COVID ⁃ 19，subjects from all provinces or municipalities of China were invited to participate in a quick questionnaire online survey on January 29th，2020. Results The effective response rate of completing questionnaire was 97.41%（3 083/3 165）. 98.54% of the subjects reported that they were very terrified. The main reasons included the high contagion（64.71%）and lack of effective treatments（19.92%）；94.45% of the subjects were concerned that they and their family members would be infected by the novel coronavirus. 99.42% knew that the virus could be transmitted from person to person；97.89% and 93.87% knew that it could spread through respiratory tract and contact respectively，97.73% knew that patients without symptom could also be contagious，96.37% knew that persons in close contact to COVID⁃19 patient were required to be quarantined for at least 14 days of medical observation. 99.09% knew that the pathogen of this disease was novel coronavirus. 65.46% knew that both medical protective masks and surgical masks could prevent COVID⁃19 effectively. 99.68% had confidence in defeating COVID⁃19，and 85.86% believed that COVID⁃19 would be controlled within the next 3 months. Study subjects mainly obtained health information through WeChat（88.97%）or websites（82.06%）. The proportions of the subjects who can cover mouth and nose when coughing or sneezing，avoid hand contacting with eyes，mouth or nose，practice hand hygiene，wear masks outside，avoid exposure to respiratory patients，and avoid the crowded were 89.85%，85.44%，95.13%，96.89%，92.18% and 96.27%，respectively. Multivariate Logistic analysis showed that gender（OR=0.544，95%CI：0.440⁃0.673，P<0.001），age（OR=1.844，95%CI：1.466⁃2.320，P<0.001），recognition （OR=2.200，95% CI：1.780 ⁃ 2.718，P<0.001） were associated with those good behaviors. Conclusion After the happened，the government and society′s vigorous publicity to the public achieved good results. The public are highly concerned and have a high awareness of the knowledge of COVID ⁃ 19. They adopt protective measures proactively. Females，middle⁃aged，and individuals with insufficient recognition are likely under⁃protected. In the different epidemic stages of the emerging infectious disease，health education should be carried out to the public based on scientific evidences.

Relationship between gross motor development and perceived motor competence in children aged 7 to 8 years old / 中国学校卫生

Objective@#To explore the relationship between gross motor development and perceived motor competence among children aged 7-8 years old.@*Methods@#Random cluster sampling method was used to conduct a test among 162 students aged 7-8(78 boys,84 girls)by using Test of Gross Motor Development Ⅲ(TGMD-3) and 3 and Pictorial Scale of Perceived competence and Social Acceptance for Young Children(PSPC-P).@*Results@#There were no statistical significance of perceived motor competence in different genders,and ages among children(t=-0.82, -0.58, P>0.05). Gross motor development differed significantly in different age groups (t=-4.79, P<0.01). Eight-year-old group(63.10±5.70) scored higher than 7-year-old group(57.96±7.76).There exists clear association between gross motor scores and perceived motor competence (r=0.29, P＜0.01), as well as locomotor development and manipulative development with perceived motor competence (r=0.21, 0.24, P<0.05).@*Conclusion@#There are close correlations between gross motor development and perceived motor competence among children aged 7 to 8 years. Manipulative development shows obvious influences on perceived motor competence. No gender differences are found in perceived motor competence.

miR-520d over-expression reverses chemotherapy resistance of TNBC via inhibiting autophagy protein Beclin1 / 中国肿瘤生物治疗杂志

@# Objective: To investigate the role and molecular mechanism of miR-520d in reversing the chemoresistance of triple negative breast cancer (TNBC) by regulating autophagy. Methods: Docetaxel (Doc) resistant cell lines MDA-MB-231/Doc and MDA-MB468/Doc were constructed by using human TNBC cell lines MDA-MB-231 and MDA-MB-468 as parental cells, and the cells were divided into blank group (parental cells), control group (drug-resistant group), and miR-520d over-expression group. The expression levels of miR-520d in cells of the blank and drug-resistant groups were detected by qPCR. The Doc-sensitivity of resistant cells over-expressing miR-520d was detected by MTT assay.After MDC staining, the generation of autophagosome in cells was observed under fluorescence microscopy; the number of miR-520d over-expressed resistant cells with positive LC3 expression was observed under confocal microscopy. The luciferase reporter gene assay was used to verify the targeting relationship between miR-520d and Beclin1. The effect of miR-520d mimics on the expression of autophagy-associated protein Beclin1, and LC3Ⅰ, LC3Ⅱ in cells was detected by WB assay. Results: The results of qPCR showed that the expression of miR-520d in the drug-resistant TNBC cells was significantly lower than that of normal cells (P<0.01). In drug-resistant cells over-expressing miR-520d, the Doc-sensitivity was significantly improved, while the autophagy activity was significantly reduced (all P<0.01).At the same time, luciferase experiments demonstrated that Beclin1 was a possible target molecule of miR-520d (P<0.05). WB results showed that the combination of docetaxel and miR-520d mimics reduced the LC3-II/I ratio and the expression of autophagy protein Beclin1 in drug-resistant TNBC cells (all P<0.05). Conclusion: The regulation of miR-520d levels may alter the expression of autophagy protein Beclin1, thereby reversing Doc chemotherapy resistance in TNBC cells.

Variants of CARD14 gene and psoriasis vulgaris in southern Chinese cohort

Abstract BACKGROUND: Recent mutation analysis identified several missense mutations in CARD14 in psoriasis. OBJECTIVES: We performed the genomic sequence analysis on CARD14 in southern Chinese Han Cantonese with Psoriasis Vulgaris (PsV) to reveal more causative missense mutations. METHODS: A total of 131 patients with PsV and 207 matched controls were included. We conducted sequence analysis of all the exon and exon-intron boundaries of CARD14 in the group of PsV patients and subsequent case control analysis of potential sequence variants of significance. RESULTS: We found five rare mutations and four of them are annotated or reported. Only the variant (c.1291C>G) has not been reported and annotated, but the variant was also found in controls. No significant difference was detected among all rare variant allele frequencies of patients and controls. CONCLUSION: None of the new definite variants were pathogenic. The other pathogenic mutations for PsV are still elusive in our cohort.

Serum metabolic profiling of human gastric cancer based on gas chromatography/mass spectrometry

Research on molecular mechanisms of carcinogenesis plays an important role in diagnosing and treating gastric cancer. Metabolic profiling may offer the opportunity to understand the molecular mechanism of carcinogenesis and help to non-invasively identify the potential biomarkers for the early diagnosis of human gastric cancer. The aims of this study were to explore the underlying metabolic mechanisms of gastric cancer and to identify biomarkers associated with morbidity. Gas chromatography/mass spectrometry (GC/MS) was used to analyze the serum metabolites of 30 Chinese gastric cancer patients and 30 healthy controls. Diagnostic models for gastric cancer were constructed using orthogonal partial least squares discriminant analysis (OPLS-DA). Acquired metabolomic data were analyzed by the nonparametric Wilcoxon test to find serum metabolic biomarkers for gastric cancer. The OPLS-DA model showed adequate discrimination between cancer and non-cancer cohorts while the model failed to discriminate different pathological stages (I-IV) of gastric cancer patients. A total of 44 endogenous metabolites such as amino acids, organic acids, carbohydrates, fatty acids, and steroids were detected, of which 18 differential metabolites were identified with significant differences. A total of 13 variables were obtained for their greatest contribution in the discriminating OPLS-DA model [variable importance in the projection (VIP) value >1.0], among which 11 metabolites were identified using both VIP values (VIP >1) and the Wilcoxon test. These metabolites potentially revealed perturbations of glycolysis and of amino acid, fatty acid, cholesterol, and nucleotide metabolism of gastric cancer patients. These results suggest that gastric cancer serum metabolic profiling has great potential in detecting this disease and helping to understand its metabolic mechanisms.